A New Reversion Assay for Measuring All Possible Base Pair
Feb 28, 2005 · REVERSION assays that can detect specific base pair substitutions have proven extremely useful. One of the best known is the set of Escherichia coli lacZ alleles that can individually detect all possible base pair substitutions (C upples and M iller 1989).
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Jul 01, 2005 · REVERSION assays that can detect specific base pair substitutions have proven extremely useful. One of the best known is the set of Escherichia coli lacZ alleles that can individually detect all possible base pair substitutions (C upples and M iller 1989).H ampsey (1991) developed a comparable tester system for yeast that can detect all possible base pair substitutions by reversion of …
A new reversion assay for measuring all possible base pair
REVERSION assays that can detect specific base pair substitutions have proven extremely useful. One of the best known is the set of Escherichia coli lacZ alleles that can individually detect all possible base pair substitutions (C upples and M iller 1989).
Cited by: 8
(a) Purpose. The Salmonella typhimurium histidine (his) reversion system is a microbial assay which measures his − → his = reversion induced by chemicals which cause base changes or frameshift mutations in the genome of this organism. (b) Definitions. (1) A reverse mutation assay in Salmonella typhimurium detects mutation in a gene of a histidine requiring strain to produce a histidine
40 CFR § 79.68
In a reversion assay, this change may occur at the site of the original mutation or at a second site in the chromosome. Frameshift mutagen is an agent which causes the addition or deletion of single or multiple base pairs in the DNA molecule.
a reversion assay based on mutations in the tetracy- cline-resistance gene (ter) of pBR322 in E. coli. The mutations, which reside in a 276-base-pair BamHI- Sal1 restriction fragment, were isolated in a forward mutation assay (Fuchs et al., 1988) or were con-
Published in: Mutation Research · 1996Authors: George R Hoffmann · Suzanne M Deschenes · Teresa Manyin · Robert P P FuchsAffiliation: College of the Holy Cross · Centre National De La Recherche ScientifiqueAbout: PBR322 · Intercalation · Base pair · Escherichia coli · Bacteria · Frameshift mutation
Lu Le Laboratory: Ames test
Aug 27, 2012 · The Ames test is a short-term assay to assess the mutagenic potential of chemical compounds. In this experiment, we use Salmonella typhimurium (His-) to be the tester, so that it is also called ” Salmonella reversion assay”.
The assay is based upon the reversion of mutations in the histidine (his) operon in the bacterium Salmonella enterica sv Typhimurium. The his operon encodes enzymes required for the biosynthesis of the amino acid histidine. Strains with mutations in the his operon are histidine auxotrophs — they are unable to grow without added histidine.
The Escherichia coli lacZ reversion mutagenicity assay
The Escherichia coli lacZ reversion assay, based on the set of episomal lacZ alleles engineered by Miller et al., provides an attractive system for studies of mutagenesis and mutational
To cite this abstract in AMA style: Thomas K, Makris A, Tsalapaki C, Lazarini A, Klavdianou K, Antonatou K, Koutsianas C, Hatzara C, Hadziyannis E, Vassilopoulos D. High Rates of Conversion and Reversion of Tuberculosis Screening Assays in Rheumatic Patients …
comparison of mutation spectra detected by the Escherichia
Jul 01, 2000 · The Lac + reversion assay was performed by the preincubation method: 0.1 ml of bacterial culture and 0.01–0.1 ml of mutagen solution were added to 0.5 ml of 100 mM sodium phosphate buffer (pH 7.4). The mixture was preincubated at 37°C for 20 min with gentle shaking and then 2 ml of molten NB top agar kept at 45°C was added.
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The Ames Test or Bacterial Reverse Mutation Test is a biological assay to assess the mutagenic potential of chemical compounds. This test is conducted in compliance with Organization for Economic Cooperation and Development (OECD) 471 Guideline for Testing of …
BRIEF REPORT Reversion and Conversion of Interferon-γ Release Assay Results in HIV-1–Infected Individuals Maximilian C. Aichelburg, 1ThomasReiberger,2 Florian Breitenecker, Mattias Mandorfer,2
The Ames Salmonella/microsome mutagenicity as-say evolved over the years from the initial screening of a number of histidine mutants which led to the selec-tion of mutants that were highly sensitive to reversion by a variety of chemical mutagens [2,5,39,40,47]. Because bacteria are unable to metabolize chemicals
Published in: Mutation Research · 2000Authors: Kristien Mortelmans · Errol ZeigerAffiliation: Sri International · National Institutes of HealthAbout: Microsome · Ames test · Microsoma · DNA damage · Toxicity · Salmonella
genes in the canavanine assay or by two-hybrid analysis (Figs. 1 and 5 and data not shown). However, the difference in mutation rate between wild-type and MMR-defective strains in the cana-vanine assay is modest (10- to 20-fold), relative to that seen in assays that measure the reversion of specific frameshift muta-tions (4, 19–22).
Therefore, it is important to elucidate the difference between Trp + reversion and His + reversion events. Salmonella typhimurium TA100 and TA1535 utilize the hisG46 missense mutation, which substitutes a proline triplet (CCC) for a leucine triplet (CTC) at position 434 of the histidine operon (Barnes et al., 1982).
d “‘ °° “‘ PNL-6873 Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Sulfur Mustard in the Salmonella Histidine Reversion Assay N Final Report 0D. L. Stewart, E. J. Sass, L. K. Fritz and L. B. Sasser (V) Pacific Northwest Laboratory, P.O. Box 999
The frequency of reversion of either IGRA test result was much higher among participants whose results were positive by only one IGRA and negative by TST and the other IGRA (isolated positive) compared with reversion of IGRA results among 30 participants with baseline triple-positive results and at …
P13 Interferon-gamma release assay (IGRA) conversion, reversion and implications for the diagnosis of latent tuberculosis infection using a multimodality approach: a retrospective, observational study within a central London TB centre
The Ames Test for mammalian environmental mutagenicity The Ames Test combines a bacterial revertant mutation assay with a simulation of mammalian metabolism to produce a highly sensitive test for mutagenic chemicals in the environment. A rat liver homogenate is prepared to produce a metabolically active extract (S9).